초록
<P>Lignocellulosic biomass (LCB) is a low-cost and abundant source of fermentable sugars. Enzymatic hydrolysis is one of the main ways to obtain sugars from biomass, but most of the polysaccharide-degrading enzymes are poorly efficient on LCB and cellulases with higher performances are required. In this study, we designed a chimeric protein by adding the carbohydrate binding module (CBM) of the cellulosomal enzyme <I>Ct</I>Lic26A-Cel5E (endoglucanase H or CelH) from <I>Clostridium (Ruminiclostridium) thermocellum</I> to the C-terminus of Dtur CelA, an interesting hyperthermostable endoglucanase from <I>Dictyoglomus turgidum</I>. The activity and binding rate of both native and chimeric enzyme were evaluated on soluble and insoluble polysaccharides. The addition of a CBM resulted in a cellulase with enhanced stability at extreme pHs, higher affinity and activity on insoluble cellulose.</P>