BioChem - DOS

Solvents derived from glycerol modify classical regioselectivity in the enzymatic synthesis of disaccharides with Biolacta β-galactosidase

Green chemistry : an international journal and green chemistry resource : GC

Pé rez-Sá nchez, Marí a; Sandoval, Manuel; Corté s-Cabrera, Alvaro; Garcí a-Marí n, Hé ctor; Sinisterra, José V.; Garcí a, José I.; Hernaiz, Marí a J.

<P>Green solvents made from glycerol change the classical regioselectivity of Biolacta N<SUP>o</SUP> 5 &beta;-galactosidase, from &beta;(1&rarr;4) to &beta;(1&rarr;6) linkages when a 2 M concentration was used. In order to explain these results, the non-proteic compounds present in the Biolacta preparation were separated by precipitation with ammonium sulfate and the remaining protein extract was used to set reactions with appropriate organic solvents to find that the regioselectivity towards the &beta;(1&rarr;6) isomer is retained. According to proteomic analysis, a 98% homology between <I>Streptococcus pneumoniae</I> and Biolacta &beta;-galactosidase preparation was found. With these data, molecular modelling was done which predicts a tridimensional interaction in the enzyme active site with the donor (GlcNAc) and the water-solvent mixture which explains this phenomenon.</P> <P>Graphic Abstract</P><P>Green solvents from glycerol change the classical regioselectivity of Biolacta &beta;-galactosidase, from &beta;(1&rarr;4) to &beta;(1&rarr;6) linkages. Molecular modelling predicts a tridimensional interaction in the enzyme active site with the donor (GlcNAc) and the water-solvent mixture which explains this phenomenon.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1gc15266a'><BR></P>

2011

Royal Society of Chemistry

1463-9262

1463-9270

13

10

pp.2810-2817

10.1039/c1gc15266a

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57551439

https://digital.csic.es/bitstream/10261/54091/5/Green%20Solvents.pdf