초록
The sono-assisted alkaline pretreatment of sugarcane bagasse was optimized using a central composite design in which the glucose recovery after enzymatic hydrolysis was used as the response function. Cellic<SUP>®</SUP> CTec3 (Novozymes) and Thermossac<SUP>®</SUP> Dry (Lallemand) were used for enzymatic hydrolysis and fermentation, respectively. The best performance was achieved at 70<SUP>o</SUP>C with 140W of sonication power using 0.125g of NaOHg<SUP>-1</SUP> of dry biomass, yielding a total glucose recovery of 95.8wt% in relation to the total glucan content of the original material. The fermentation efficiency of the best substrate hydrolysate was 91.4%, whereas lower values of 87.1 and 80.0% were obtained for steam-exploded and sono-assisted alkali-washed steam-exploded cane bagasse, respectively. In addition, when the glucan recovery of each pretreatment method was taken into account, the total C6 cellulosic ethanol production from the sono-assisted alkali-washed cane bagasse was 20.4 and 40.6% higher than that of the other two pretreatment technologies, respectively.