BioChem - DOS

Engineering Yarrowia lipolytica as a Cellulolytic Cell Factory for Production of p-Coumaric Acid from Cellulose and Hemicellulose

Journal of agricultural and food chemistry

Zhu, Jiarui; Yang, Shu; Cao, Qing; Li, Xiaoyan; Jiao, Liangcheng; Shi, Yuanxing; Yan, Yunjun; Xu, Li; Yang, Min; Xie, Xiaoman; Madzak, Catherine; Yan, Jinyong

<P><I>De novo</I> biosynthesis of high-value added food additive <I>p</I>-coumaric acid (<I>p</I>-CA) direct from cellulose/hemicellulose is a more sustainable route compared to the chemical route, considering the abundant cellulose/hemicellulose resources. In this study, a novel factory was constructed for the production of <I>p</I>-CA in <I>Yarrowia lipolytica</I> using cellulose/hemicellulose as the sole carbon source. Based on multicopy integration of the <I>TAL</I> gene and reprogramming the shikimic acid pathway, the engineered strain produced 1035.5 ± 67.8 mg/L <I>p</I>-CA using glucose as a carbon source. The strains with overexpression of cellulases and hemicellulases produced 84.3 ± 2.4 and 65.3 ± 4.6 mg/L <I>p</I>-CA, using cellulose (carboxymethyl-cellulose) or hemicellulose (xylan from bagasse) as the carbon source, respectively. This research demonstrated the feasibility of conversion of cost-effective cellulose/hemicellulose into a value-added product and provided a sustainable cellulolytic cell factory for the utilization of cellulose/hemicellulose.</P><BR>[FIG OMISSION]</BR>

2024

American Chemical Society

0021-8561

1520-5118

72

11

pp.5867-5877

10.1021/acs.jafc.4c00567

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART129270759

cellulose; hemicellulose; Yarrowia lipolytica; p-coumaric acid; metabolic engineering