BioChem - DOS

Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability

Metabolic engineering

Zhu, L.; Dong, H.; Zhang, Y.; Li, Y.

To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C. acetobutylicum DSM1731 by cloning and over-expressing the gshAB genes from Escherichia coli. Strain DSM1731(pITAB) produces glutathione, and shows a significantly improved survival upon aeration and butanol challenge, as compared with the control. In addition, strain DSM1731(pITAB) exhibited an improved butanol tolerance and an increased butanol production capability, as compared with the recombinant strains with only gshA or gshB gene. These results illustrated that introducing glutathione biosynthetic pathway, which is redundant for the metabolism of C. acetobutylicum, can increase the robustness of the host to achieve a better solvent production.

2011

Academic Press

1096-7176

1096-7184

13

4

pp.426-434

10.1016/j.ymben.2011.01.009

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56531510

Clostridium acetobutylicum; Robustness; Glutathione; Aero-tolerance; Butanol tolerance; Butanol production