BioChem - DOS

Enzymatic hydrolysis of ionic liquid-pretreated celluloses: Contribution of CP-MAS 13C NMR and SEM

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Husson, E.; Buchoux, S.; Avondo, C.; Cailleu, D.; Djellab, K.; Gosselin, I.; Wattraint, O.; Sarazin, C.

The supramolecular structure of four model celluloses was altered prior to their enzymatic saccharification using two ionic liquid pretreatments: one with the commonly used 1-ethyl-3-methylimidazolium acetate ([Emim]<SUP>+</SUP>[CH<SUB>3</SUB>COO]<SUP>-</SUP>) and the other with the newly developed 1-ethyl-3-methylimidazolium methylphosphonate ([Emim]<SUP>+</SUP>[MeO(H)PO<SUB>2</SUB>]<SUP>-</SUP>). The estimation of crystallinity index (CrI) by solid state <SUP>13</SUP>C nuclear magnetic resonance for each untreated/pretreated celluloses was compared with the performances of their enzymatic hydrolysis. For &alpha;-cellulose, both pretreatments led to a significant decrease in CrI from 25% to 5% but had no effect on glucose yields. In contrast, The [Emim]<SUP>+</SUP>[MeO(H)PO<SUB>2</SUB>]<SUP>-</SUP> pretreatment on the long fibers of cellulose had no significant effect on the CrI although a conversion yield in glucose of 88% is obtained versus 32% without pretreatment. However, scanning electron microscopy analysis suggested a loss of fiber organization induced by both ionic liquid pretreatments leading to a larger accessibility by cellulases to the cellulose surface.

2011

Elsevier Applied Science

0960-8524

102

15

pp.7335-7342

10.1016/j.biortech.2011.04.097

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56534172

Cellulose; Ionic liquid; Cellulase from Trichoderma reesei; Supramolecular structure; Crystallinity