BioChem - DOS

Fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enhancing enzymatic saccharification and hydrogen production

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zhao, L.; Cao, G.L.; Wang, A.J.; Ren, H.Y.; Dong, D.; Liu, Z.N.; Guan, X.Y.; Xu, C.J.; Ren, N.Q.

The feasibility of fungal pretreatment of cornstalk with Phanerochaete chrysosporium for enzymatic saccharification and H<SUB>2</SUB> production was investigated in this study. Firstly, cornstalk was pretreated with P. chrysosporium at 29<SUP>o</SUP>C under static condition for 15d, lignin reduction was up to 34.3% with holocellulose loss less than 10%. Microscopic structure observation combined FTIR analysis further demonstrated that the lignin and crystallinity were decreased. Subsequently, the fungal-pretreated cornstalk was subjected to enzymatic hydrolysis by the crude cellulase from Trichoderma viride to produce fermentable sugars which were then fermented to bio-H<SUB>2</SUB> using Thermoanaerobacterium thermosaccharolyticum W16. The maximum enzymatic saccharification was found to be 47.3% which was 20.3% higher than the control without pretreatment. Upon fermentation of enzymatic hydrolysate, the yield of H<SUB>2</SUB> was calculated to be 80.3ml/g-pretreated cornstalk. The present results suggested the potential of using hydrogen-producing bacteria for high-yield conversion of cornstalk into bio-H<SUB>2</SUB> integrate with biological pretreatment and enzymatic saccharification.

2012

Elsevier Applied Science

0960-8524

114

pp.365-369

10.1016/j.biortech.2012.03.076

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART62358091

Fungal pretreatment; Phanerochaete chrysosporium; Saccharification; Hydrogen production