초록
<P><B>Abstract</B></P><P><I>The search for renewable energies has been stimulating the production of ethanol, which can be produced by using agro-industrial waste as substrates for fermentation. The aim of this investigation was to use whey permeate and sugarcane molasses to obtain bioethanol by submerged fermentation using the thermotolerant yeast</I> Kluyveromyces marxianus<I>. The concentrations of ethanol and sugars were determined by high performance liquid chromatography. The highest ethanol concentration of 50.04 g/L was obtained with the optimized medium with 100, 6.26,</I><I>and 6.26 g/L of lactose (from whey permeate), yeast extract, and (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>, respectively. The association of whey permeate (WP) with sugarcane molasses (SM) in the fermentation medium led to higher concentrations of ethanol (49.11 g/L) in the proportion of 80% WP and 20% SM. Therefore, the use of the agroindustrial residues in fermentation suggests a viable industrial application and can generate advantages for being low-cost by-products.</I></P>