BioChem - DOS

Improved polyhydroxybutyrate (PHB) production in transgenic tobacco by enhancing translation efficiency of bacterial PHB biosynthetic genes

Journal of bioscience and bioengineering

Matsumoto, Ken'ichiro; Morimoto, Kenjiro; Gohda, Aoi; Shimada, Hiroaki; Taguchi, Seiichi

<P><B>Abstract</B></P><P>Polyhydroxybutyrate [P(3HB)] was produced in the transgenic tobacco harboring the genes encoding acetoacetyl-CoA reductase (PhaB) and polyhydroxyalkanoate synthase (PhaC) from <I>Ralstonia eutropha</I> (<I>Cupriavidus necator</I>) with optimized codon usage for expression in tobacco. P(3HB) contents in the transformants (0.2mg/g dry cell weight in average) harboring the codon-optimized <I>phaB</I> gene was twofold higher than the control transformants harboring the wild-type <I>phaB</I> gene. The immunodetection revealed an increased production of PhaB in leaves, indicating that the enhanced expression of PhaB was effective to increase P(3HB) production in tobacco. In contrast, codon-optimization of the <I>phaC</I> gene exhibited no apparent effect on P(3HB) production. This result suggests that the efficiency of PhaB-catalyzed reaction contributed to the flux toward P(3HB) biosynthesis in tobacco leaves.</P>

2011

Elsevier

1389-1723

1347-4421

111

4

pp.485-488

10.1016/j.jbiosc.2010.11.020

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55942399

https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/45613/1/JBB111-4_485-488.pdf

Transgenic plant; Nicotiana tabacum; PHA synthase; Real-time PCR