초록
<P><B>Abstract</B></P> <P>In this study, distillery wastewater was treated by dark fermentation or photofermentation alone, and by sequential dark and photofermentation processes using anaerobic saccharolytic consortium and purple nonsulfur bacteria. Combination of dark and photofermentation resulted in the maximal H<SUB>2</SUB> yield of 17.6L/L of distillery waste with chemical oxygen demand 40g/L. It is equivalent to 205kJ/L distillery wastewater and corresponds to recovery of approximately 4–8% of energy consumed during ethanol production. Optimal performance of photofermentation was observed at 20% concentration of pre-fermented distillery waste. In photofermentation, the range of the suitable distillery waste concentrations was extended and the H<SUB>2</SUB> yield was improved by choosing the tolerant strain of purple bacteria <I>Rhodobacter sphaeroides</I> B-3059. After two stages, organic acids and sugars were completely consumed that means wastewater treatment concomitant to H<SUB>2</SUB> production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 17.6L H<SUB>2</SUB> can be produced from 1L of distillery waste by dark+photo fermentation. </LI> <LI> An estimated 4–8% of energy consumed in ethanol production might be recovered as H<SUB>2</SUB>. </LI> <LI> Integrated process resulted in wastewater purification from sugars and VFAs. </LI> <LI> Further efforts should be directed to optimize the scale-up process. </LI> </UL> </P>