초록
<P><B>Abstract</B></P> <P>The biological conversion of carbon monoxide (CO) has been highlighted for the development of a C1 gas biorefinery process. Despite this, the toxicity and low reducing equivalent of CO uptake make biological conversion difficult. The use of synthetic co-cultures is an alternative way of enhancing the performance of CO bioconversion. This study evaluated a synthetic co-culture consisting of <I>Citrobacter amalonaticus</I> Y19 and <I>Sporomusa ovata</I> for acetate production from CO. In this consortium, the CO<SUB>2</SUB> and H<SUB>2</SUB> produced by the water-gas shift reaction of <I>C. amalonaticus</I> Y19, were utilized further by <I>S. ovata</I>. Higher acetate production was achieved in the co-culture system compared to the monoculture counterparts. Furthermore, syntrophic cooperation via various reducing equivalent carriers provided new insights into the synergistic metabolic benefits with a toxic and refractory substrate, such as CO. This study also suggests an appropriate model for examining the syntrophic interaction between microbial species in a mixed community.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-culture of <I>C. amalonaticus</I> Y19 and <I>S. ovata</I> was examined using CO as substrate. </LI> <LI> CO was converted to CO<SUB>2</SUB> and H<SUB>2</SUB> by Y19, then metabolized by ovata. </LI> <LI> Co-culture presented better CO consumption and acetate production than mono-culture. </LI> <LI> The addition of HNQ as mediator improves conversion of CO to acetate. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>