Cellulase production, simultaneous saccharification and fermentation in a single vessel: A new approach for production of bio-ethanol from mild alkali pre-treated water hyacinth
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Cellulase production, simultaneous saccharification and fermentation in a single vessel: A new approach for production of bio-ethanol from mild alkali pre-treated water hyacinth
<P><B>Abstract</B></P> <P>The current paper reports on-site cellulase production followed by simultaneous saccharification and fermentation (SSF) of pre-treated water hyacinth for ethanol production in the same flask. Pre-treatment was carried out with 0.5% (w/v) NaOH at room temperature (RT) and 121°C for 24h and 30min, respectively. SSF studies were performed with <I>Kluveromyces marxianus</I> (42°C) and <I>Saccharomyces cerevisae</I> (30°C) at 8% solids loading (w/v). RT and 121°C pre-treated water hyacinth yielded maximum ethanol concentration of 7.32, 8.04 and 5.21, 5.98gL<SUP>−1</SUP> after 48h at 42 and 30°C, respectively. On 8th day, crude cellulases were supplemented with commercial pectinase. Supplementation of pectinase yielded 1.04 to 1.11 fold higher ethanol yields as compared to without pectinase flasks. The current ethanol yields were achieved at higher solid loadings in a single flask by avoiding number of steps/flasks as required in the conventional process.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bio-ethanol production from water hyacinth in a single reactor. </LI> <LI> Inverted cone frustum type in-house designed pretreatment reactor. </LI> <LI> Enhanced ethanol yields by addition of pectinase from water hyacinth. </LI> </UL> </P>