BioChem - DOS

Biocatalytic reduction of prochiral aromatic ketones to optically pure alcohols by a coupled enzyme system for cofactor regeneration

Tetrahedron letters: the international organ for the rapid publication of preliminary communications in organic chemistry

Yan, Zhen; Nie, Yao; Xu, Yan; Liu, Xiang; Xiao, Rong

<P><B>Graphical abstract</B></P><P><ce:figure id='f0020'></ce:figure> <P><B>Abstract</B></P><P>A simple, highly efficient, and economical biphasic cell-free system was developed for biocatalytic reduction of prochiral aromatic ketones to furnish enantiopure alcohols. This system is characterized by using endogenous enzymes of the cell-free extract to form enzyme-coupled NADPH recycling system. Besides, it offered much higher productivity than whole cells and greatly simplified the preparation process of biocatalysts in comparison with isolated enzymes. Various prochiral aromatic ketones, especially &alpha;-substituted acetophenone derivatives, were reduced to chiral alcohols with excellent enantiomeric excess (ee) and moderate to good yield by this cell-free system.</P>

2011

0040-4039

1873-3581

52

9

pp.999-1002

10.1016/j.tetlet.2010.12.069

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55267696

Asymmetric carbonyl reduction; Cofactor regeneration; Enzyme-coupled system; Cell-free extract