Improvement of Hydrogen Productivity by Introduction of NADH Regeneration Pathway in Clostridium paraputrificum

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논문

Improvement of Hydrogen Productivity by Introduction of NADH Regeneration Pathway in Clostridium paraputrificum

학술지

Applied biochemistry and biotechnology

저자명

Lu, Yuan; Zhang, Chong; Zhao, Hongxin; Xing, Xin-Hui

초록

<P>To improve the hydrogen productivity and examine the hydrogen evolution mechanism of Clostridium paraputrificum, roles of formate in hydrogen evolution and effects of introducing formate-originated NADH regeneration were explored. The formate-decomposing pathway for hydrogen production was verified to exist in C. paraputrificum. Then NAD(+)-dependent formate dehydrogenase FDH1 gene (fdh1) from Candida boidinii was overexpressed, which regenerate more NADH from formate to form hydrogen by NADH-mediated pathway. With fdh1 overexpression, the hydrogen yield via NADH-involving pathway increased by at least 59 % compared with the control. Accompanied by the change of hydrogen metabolism, the whole cellular metabolism was redistributed greatly.</P>

발행연도

2012

발행기관

Springer-Verlag

ISSN

0273-2289

ISSN

1559-0291

권

167

호

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