BioChem - DOS

Flux balance analysis of mixed anaerobic microbial communities: Effects of linoleic acid (LA) and pH on biohydrogen production

International journal of hydrogen energy

Chaganti, S.R.; Kim, D.H.; Lalman, J.A.

The internal fluxes of mixed anaerobic cultures fed 2000 mg l<SUP>-1</SUP> linoleic acid (LA) plus glucose at 6 initial pH conditions and maintained at 37 <SUP>o</SUP>C were estimated using a flux balanced analysis (FBA). In cultures fed LA at pH 7, less than 8% of the flux was diverted to CH<SUB>4</SUB>. At an initial pH >= 5.5, the quantity of glucose removed was greater than 95%; however, at pH 4.5 and 5.0 the quantity consumed were 38% and 75%, respectively. The FBA output showed that the acetogenic H<SUB>2</SUB>-consumers were responsible for more than 20% of the H<SUB>2</SUB> consumed. Adding LA and decreasing the pH was ineffective in reducing the activity of acetogenic H<SUB>2</SUB>-consumers. As the initial pH decreased, the acetogenic H<SUB>2</SUB>-consuming flux decreased in the presence of 2000 mg l<SUP>-1</SUP> LA. A maximum H<SUB>2</SUB> yield of 1.55 mol mol<SUP>-1</SUP> glucose consumed (peak hydrogenase flux (R12)) was attained when the acetogenic H<SUB>2</SUB>-consuming flux reached 0.42 mol at a pH of 5.5.

2011

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

36

21

pp.14141-14152

10.1016/j.ijhydene.2011.04.161

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57433427

Flux balance analysis; Mixed culture; Anaerobic; Hydrogen fermentation; Acetogenic H2-consumers; Universal bacterium