초록
<P><B>Abstract</B></P> <P>Lipid was produced by <I>Rhodosporidiobolus fluvialis</I> DMKU-SP314 from a mixture of low-cost substrates: sugarcane top hydrolysate (STH) and biodiesel-derived crude glycerol. The optimized lipid production medium for shaking flask cultivation contained STH supplemented with 59g/L crude glycerol, 0.21g/L soybean powder, 0.9g/L (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>, 0.4g/L KH<SUB>2</SUB>PO<SUB>4</SUB>, 2.0g/L MgSO<SUB>4</SUB>·7H<SUB>2</SUB>O and pH 6.1 (C/N ratio 60), while the optimal cultivation temperature was 28°C. Batch cultivation in a 2L stirred-tank fermenter at an agitation speed of 300rpm and an aeration rate of 2vvm achieved the maximum lipid quantity of 18.2g/L, which was 75.0% of dry biomass (24.3g/L biomass), after 240h of cultivation. These represent a 5.4-fold and 1.7-fold improvement in the lipid concentration and lipid content, respectively, compared to non-optimized shaking flask cultivation. Under optimal conditions, the lipid produced had a high monounsaturated fatty acid content (36.6% of oleic acid), which is suitable for biodiesel production. Thus, <I>R. fluvialis</I> DMKU-SP314 is capable of producing large quantities of lipid from low-cost raw materials in a process that can be readily scaled up for industrial production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lipid production by oleaginous red yeast, <I>R. fluvialis</I> DMKU-SP314, was optimized. </LI> <LI> DMKU-SP314 accumulate high lipid in sugarcane top hydrolysate and crude glycerol. </LI> <LI> Lipid production in a 2L fermenter achieved high lipid content of 75%. </LI> <LI> DMKU-SP314 has potential for lipid production from low-cost substrates. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>