<P><B>Abstract</B></P> <P>The aim of this study was to develop an integrated biofuel (cellulosic bioethanol) and biochemical (succinic acid) production process from rapeseed straw after dilute-acid pretreatment. Rapeseed straw pretreatment at 20% (w/v) solid loading and subsequent hydrolysis with Cellic® CTec2 resulted in high glucose yield (80%) and ethanol output (122–125 kg of EtOH/Mg of rapeseed straw). Supplementation the enzymatic process with 10% dosage of endoxylanases (Cellic® HTec2) reduced the hydrolysis time required to achieve the maximum glucan conversion by 44–46% and increased the xylose yield by 10% compared to the process with Cellic® CTec2. Significantly higher amounts of succinic acid were produced after fermentation of pretreatment liquor (48 kg/Mg of rapeseed straw, succinic acid yield: 60%) compared to fermentation of xylose-rich residue after ethanol production (35–37 kg/Mg of rapeseed straw, succinic yield: 68–71%). Results obtained in this study clearly proved the biorefinery potential of rapeseed straw.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Ethanol and succinic acid production from rape straw in a biorefinery approach. </LI> <LI> 20% biomass loading during pretreatment and Cellic® CTec2 ensured effective sugar conversion. </LI> <LI> Endoxylanases supplementation (Cellic® HTec2) increased xylose yield by 10%. </LI> <LI> Succinic acid effectively produced from residues after straw processing into bioethanol. </LI> </UL> </P>