BioChem - DOS

Systems pathway engineering of Corynebacterium crenatum for improved L-arginine production

Scientific reports

Man, Zaiwei; Xu, Meijuan; Rao, Zhiming; Guo, Jing; Yang, Taowei; Zhang, Xian; Xu, Zhenghong

<P>L-arginine is an important amino acid in food and pharmaceutical industries. Until now, the main production method of L-arginine in China is the highly polluting keratin acid hydrolysis. The industrial level L-arginine production by microbial fermentation has become an important task. In previous work, we obtained a new L-arginine producing <I>Corynebacterium crenatum</I> (subspecies of <I>Corynebacterium glutamicum</I>) through screening and mutation breeding. In this work, we performed systems pathway engineering of <I>C. crenatum</I> for improved L-arginine production, involving amplification of L-arginine biosynthetic pathway flux by removal of feedback inhibition and overexpression of arginine operon; optimization of NADPH supply by modulation of metabolic flux distribution between glycolysis and pentose phosphate pathway; increasing glucose consumption by strengthening the preexisting glucose transporter and exploitation of new glucose uptake system; channeling excess carbon flux from glycolysis into tricarboxylic acid cycle to alleviate the glucose overflow metabolism; redistribution of carbon flux at &alpha;-ketoglutarate metabolic node to channel more flux into L-arginine biosynthetic pathway; minimization of carbon and cofactor loss by attenuation of byproducts formation. The final strain could produce 87.3 g L<SUP>&#x2212;1</SUP> L-arginine with yield up to 0.431 g L-arginine g<SUP>&#x2212;1</SUP> glucose in fed-batch fermentation.</P>

2016

Nature Publishing Group

cc-by

2045-2322

6

pp.28629

10.1038/srep28629

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75913622

https://www.nature.com/articles/srep28629.pdf