초록
A newly isolated photosynthetic bacterium, Rhodobacter sphaeroides HY01 showed high hydrogen production performance and good flexibility on genetic modification. In order to investigate its potential on using wastewater containing heavy metal ions for photosynthetic hydrogen production, the effects of four conventional heavy metal ions, Pb<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Cr<SUP>6+</SUP> and Cd<SUP>2+</SUP>, on the photosynthetic hydrogen production performance of R. sphaeroides HY01 were studied. The results suggested that the concentration of Pb<SUP>2+</SUP> 0.5 mg/L, Cu<SUP>2+</SUP> 0.05 mg/L, Cr<SUP>6+</SUP> 0.05 mg/L, Cd<SUP>2+</SUP> 20 mg/L showed maximum positive effects on the hydrogen production rates and yields. This strain cultured in different heavy metal ions at optimum concentrations showed similar growth curves and optical density. The removal efficiency of heavy metal ions were determined to be 65%-76% for Cd<SUP>2+</SUP>, 54%-80% for Cr<SUP>6+</SUP>, 30%-38% for Pb<SUP>2+</SUP>and 96%-100% for Cu<SUP>2+</SUP>. The results suggest that HY01 was sensitive to Cr<SUP>6+</SUP> and Pb<SUP>2+</SUP>, and relative of high tolerance to Cu<SUP>2+</SUP> and Cd<SUP>2+</SUP>.