초록
<P><B>Highlights</B></P><P>► Glycerin and casein were the best for algae UTEX2341 biomass and lipid production. ► Biomass and lipid yields were 28.7 and 11.9 times higher than that by Illman and Oh. ► The unsaturated fatty acids were the main parts of FAME. ► 94% of the fatty acid was composed of the palmitic, oleic, linoleic and γ linoleic.</P> <P><B>Abstract</B></P><P>High production cost is a major obstacle to the extensive use of microalgae biodiesel. To cut the cost and achieve higher biomass productivity, <I>Chlorella minutissima</I> UTEX2341 was cultured under photoheterotrophic conditions. With the carbon, nitrogen and phosphorus concentration of 26.37, 2.61 and 0.03gL<SUP>−1</SUP>d<SUP>−1</SUP> respectively, a maximum biomass productivity of 1.78gL<SUP>−1</SUP>d<SUP>−1</SUP> was obtained, which was 59 times more than that cultured under autotrophic condition. The lipid productivity reached 0.29gL<SUP>−1</SUP>d<SUP>−1</SUP>, which was 11.9 times higher than the highest value reported by <ce:cross-ref refid='b0135'>Oh et al. (2010)</ce:cross-ref>. The conversion rate of microalgae lipids to FAME was found to be elevated from 45.65% to 62.97% and the FAME productivity increased from 1.16 to 180.68mgL<SUP>−1</SUP>d<SUP>−1</SUP> after the optimization. 94% of the fatty acid of <I>C. minutissima</I> UTEX2341 was found to be composed of palmitic, oleic, linoleic and γ linoleic and the unsaturated fatty acids were the main parts (79.42%).</P>