BioChem - DOS

Enhancing enzymatic hydrolysis of coconut husk through Pseudomonas aeruginosa AP 029/GLVIIA rhamnolipid preparation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

de Araú jo, Cynthia Ké rzia Costa; de Oliveira Campos, Alan; de Araú jo Padilha, Carlos Eduardo; de Sousa Jú nior, Francisco Canindé do Nascimento, Ruthiné ia Jé ssica Alves; de Macedo, Gorete Ribeiro; dos Santos, Everaldo Silvino

<P><B>Abstract</B></P> <P>This work investigated the influence of chemical (Triton X-100) and biological surfactant preparation (rhamnolipids) in coconut husk hydrolysis that was subjected to pretreatment with acid-alkali or alkaline hydrogen peroxide. The natural and pretreated biomass was characterized using the National Renewable Energy Laboratory protocol analysis as well as X-ray diffraction and scanning electron microscopy. The results demonstrated that in terms of the total reducing sugars, there was no significant difference between the hydrolysis using Triton X-100 and rhamnolipids, regardless of the pretreatment. A cellulosic conversion value as high as 33.0% was obtained in experiments with rhamnolipids. The coconut husk was observed to be a potential biomass that could produce second generation ethanol, and the rhamnolipid preparation can be used to support for the enzymatic hydrolysis, enhancing the advantage of cellulose conversion into glucose over chemical surfactants because it is an environmentally friendly approach.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lignocellulosic coconut husk was pre-treated by acid-alkaline and alkaline hydrogen peroxide. </LI> <LI> Rhamnolipids and the Triton X-100 influenced the enzymatic hydrolysis. </LI> <LI> Rhamnolipids could enhance cellulosic conversion of coconut husk by 33.0%. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

0960-8524

237

pp.20-26

10.1016/j.biortech.2017.03.178

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77891339

Lignocellulosic; Cellulases; Hydrolysis; Coconut husk; Rhamnolipids; Triton X-100