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Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

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논문

Invertase, glucose oxidase and catalase for converting sucrose to fructose and gluconic acid through batch and membrane-continuous reactors

학술지

Brazilian journal of pharmaceutical sciences : BJPS

저자명

Silva, Aline Ramos da; Tomotani, Ester Junko; Vitolo, Michele

초록

<▼1><P>Conversion of sucrose into fructose and gluconic acid using invertase, glucose oxidase and catalase was studied by discontinuous (sequential or simultaneous addition of the enzymes) and continuous (simultaneous addition of the enzymes in a 100 kDa-ultrafiltration membrane reactor) processes. The following parameters were varied: concentration of enzymes, initial concentration of substrates (sucrose and glucose), pH, temperature and feeding rate (for continuous process). The highest yield of conversion (100%) was attained through the discontinuous (batch) process carried out at pH 4.5 and 37 ºC by the sequential addition of invertase (14.3 U), glucose oxidase (10,000 U) and catalase (59,000 U).</P></▼1><▼2><P>Neste trabalho estudou-se a convers&atilde;o da sacarose em frutose e &aacute;cido glic&ocirc;nico, usando as enzimas invertase, glicose oxidase e catalase, atrav&eacute;s do emprego de processo descont&iacute;nuo (com adi&ccedil;&atilde;o sequencial ou simult&acirc;nea das enzimas) e cont&iacute;nuo (adi&ccedil;&atilde;o simult&acirc;nea das enzimas em reator com membrana acoplado &agrave; membrana de ultrafiltra&ccedil;&atilde;o de 100 kDa). Os par&acirc;metros variados foram: a concentra&ccedil;&atilde;o das enzimas, a concentra&ccedil;&atilde;o inicial dos substratos (sacarose e glicose), o pH, a temperatura e a vaz&atilde;o espec&iacute;fica de alimenta&ccedil;&atilde;o (processo cont&iacute;nuo). Obteve-se rendimento de 100%, quando a convers&atilde;o foi conduzida por processo descont&iacute;nuo em pH 4,5 e a 37 ºC com adi&ccedil;&atilde;o seq&uuml;encial das enzimas invertase (14,3 U), glicose oxidase (10.000 U) e catalase (59.000 U).</P></▼2>

발행연도

2011

발행기관

SciELO

라이선스

cc-by

ISSN

1984-8250

ISSN

2175-9790

47

2

페이지

pp.399-407

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1 2023-12-11
3 2023-12-11

논문; 2011-06-01

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