Biotransformation and improved enzymatic extraction of chlorogenic acid from coffee pulp by filamentous fungi

BioChem - DOS

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논문

Biotransformation and improved enzymatic extraction of chlorogenic acid from coffee pulp by filamentous fungi

학술지

Biotechnology progress

저자명

Torres-Mancera, Marí a Teresa; Baqueiro-Peñ a, Itzamná Figueroa-Montero, Arturo; Rodrí guez-Serrano, Gabriela; Gonzá lez-Zamora, Eduardo; Favela-Torres, Ernesto; Saucedo-Castañ eda, Gerardo

초록

<P>The highest enzymatic extraction of covalent linked chlorogenic (36.1%) and caffeic (CA) (33%) acids from coffee pulp (CP) was achieved by solid-state fermentation with a mixture of three enzymatic extracts produced by Aspergillus tamarii, Rhizomucor pusillus, and Trametes sp. Enzyme extracts were produced in a practical inexpensive way. Synergistic effects on the extraction yield were observed when more than one enzyme extract was used. In addition, biotransformation of chlorogenic acid (ChA) by Aspergillus niger C23308 was studied. Equimolar transformation of ChA into CA and quinic acids (QA) was observed during the first 36 h in submerged culture. Subsequently, after 36 h, equimolar transformation of CA into protocatechuic acid was observed; this pathway is being reported for the first time for A. niger. QA was used as a carbon source by A. niger C23308. This study presents the potential of using CP to produce enzymes and compounds such as ChA with biological activities.</P>

발행연도

2013

발행기관

Wiley (John WileySons)

ISSN

8756-7938

ISSN

1520-6033

권

호

페이지

pp.337-345