BioChem - DOS

Enzyme-mediated nutrient release: glucose-precursor activation by β-galactosidase to induce bacterial growth

Organic & biomolecular chemistry

Karton-Lifshin, Naama; Vogel, Uwe; Sella, Eran; Seeberger, Peter H.; Shabat, Doron; Lepenies, Bernd

<P>Bacteria will gain an advantage if they are able to metabolize nutrients that are inaccessible for other bacteria. To demonstrate this principle, we developed a simple model system, which mimics how bacteria exploit natural carbon sources. A masked glucose precursor that is activated by &beta;-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium. No bacterial growth was observed in the presence of control substances in which &beta;-galactosidase mediated cleavage did not lead to glucose release. This study represents a proof-of-principle example in which a bacterium can grow in a nutrient-free medium by inducible, enzyme-mediated nutrient release from a precursor.</P><BR><BR><P>Graphic Abstract</P><P>A masked glucose precursor that is activated by &beta;-galactosidase was used as a carbon source for bacterial growth in a glucose-deficient medium.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3ob27385g'><BR></P>

2013

The Royal Society of Chemistry

1477-0520

1477-0539

11

17

pp.2903-2910

10.1039/c3ob27385g

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65756230