The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
The genetic basis of variation in clean lineages of Saccharomyces cerevisiae in response to stresses encountered during bioethanol fermentations
학술지
PloS one
저자명
Greetham, Darren; Wimalasena, Tithira T.; Leung, Kay; Marvin, Marcus E.; Chandelia, Yogeshwar; Hart, Andrew J.; Phister, Trevor G.; Tucker, Gregory A.; Louis, Edward J.; Smart, Katherine A.
초록
<P><I>Saccharomyces cerevisiae</I> is the micro-organism of choice for the conversion of monomeric sugars into bioethanol. Industrial bioethanol fermentations are intrinsically stressful environments for yeast and the adaptive protective response varies between strain backgrounds. With the aim of identifying quantitative trait loci (QTL's) that regulate phenotypic variation, linkage analysis on six F1 crosses from four highly divergent clean lineages of <I>S. cerevisiae</I> was performed. Segregants from each cross were assessed for tolerance to a range of stresses encountered during industrial bioethanol fermentations. Tolerance levels within populations of F1 segregants to stress conditions differed and displayed transgressive variation. Linkage analysis resulted in the identification of QTL's for tolerance to weak acid and osmotic stress. We tested candidate genes within loci identified by QTL using reciprocal hemizygosity analysis to ascertain their contribution to the observed phenotypic variation; this approach validated a gene (<I>COX20</I>) for weak acid stress and a gene (<I>RCK2</I>) for osmotic stress. Hemizygous transformants with a sensitive phenotype carried a <I>COX20</I> allele from a weak acid sensitive parent with an alteration in its protein coding compared with other <I>S. cerevisiae</I> strains. <I>RCK2</I> alleles reveal peptide differences between parental strains and the importance of these changes is currently being ascertained.</P>