초록
Bacterial cellulose production by Acetobacter xylinum BPR 2001 was optimized using maple syrup as a carbon source. Twelve culture parameters were screened by the Plackett-Burman design and significant parameters were optimized by the response surface methodology using a three-level, four-factor Box-Behnken design. For fermentation in a rotary shaker, the optimal conditions for bacterial cellulose production were: maple syrup 30gcarbohydrate/l, (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB> 3.3g/l, KH<SUB>2</SUB>PO<SUB>4</SUB> 1g/l, yeast extract 20g/l, citric acid 1.6g/l, trisodium citrate dehydrate 2.4g/l, ethanol 0.5% (v/v), acetic acid 0.5g/l, MgSO<SUB>4</SUB>.7H<SUB>2</SUB>O 0.8g/l, inoculum age 3 days, inoculum volume 6% (v/v), shaking speed 135rpm, and incubation temperature 25<SUP>o</SUP>C. Comparison of bacterial cellulose production with maple syrup or pure sugars showed maple syrup was a suitable carbon source. This was the first demonstration of conversion of maple syrup, a plentiful renewable resource, into bacterial cellulose, a nanobiomaterial ideal for many applications.