BioChem - DOS

High efficient expression of cellobiase gene from Aspergillus niger in the cells of Trichoderma reesei

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Wang, B.; Xia, L.

The cellobiase gene from Aspergillus niger was cloned and connected with the strong promoter Pcbh1 from Trichoderma reesei to construct a recombinant plasmid pHB9 with the hygromycin B resistance marker. The plasmid was transformed into conidia of T. reesei using the modified PEG-CaCl<SUB>2</SUB> method. Main factors effecting the transformation were discussed and about 99-113transformants/&mu;g DNA could be obtained under optimal conditions. It was found that the molecular mass of the recombinant cellobiase was about 120kDa by SDS-PAGE analysis. The activity of cellobiase could reach 5.3IU/ml after 48h fermentation, which was as high as 106 times compared with that of the host strain. Meanwhile, the filter paper activity of recombinant T. reesei was 1.44-fold of the host strain. Saccharification of corncob residue with the crude enzyme showed that the hydrolysis yield (84.2%) of recombinant T. reesei was 21% higher than that (69.5%) of the host strain.

2011

Elsevier Applied Science

0960-8524

102

6

pp.4568-4572

10.1016/j.biortech.2010.12.099

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55435317

Cellobiase; Aspergillus niger; Transformation; Trichoderma reesei; Gene expression