초록
<P>Escherichia coli DH5α strain was selected as the recombinant host, and a chemically defined medium supplemented with amino acids was used instead of a complex medium for the efficient production of β-carotene. In a fed-batch culture using glycerol with a chemically defined medium supplemented with amino acids, the concentration, specific content, and productivity of β-carotene were 2,470?mg/l, 72?mg/g cells, and 77?mg/l?h after 32?h, respectively. These values were, respectively, 43, 33, and 26?% higher than those obtained using the complex medium. This is the highest β-carotene production that has been reported among the recombinant cells to date.</P>