초록
<P>We report an enzymatic route for the formal regio- and stereoselective aminohydroxylation of β-methylstyrene that consumes only dioxygen, ammonia and formate; carbonate is the by-product. The biocascade entails highly selective epoxidation, hydrolysis and hydrogen-borrowing alcohol amination. Thus, β-methylstyrene was converted into 1<I>R</I>,2<I>R</I> and 1<I>S</I>,2<I>R</I>-phenylpropanolamine in 59-63% isolated yields, and up to >99.5 : <0.5 dr and er.</P><P>Graphic Abstract</P><P>A multi-enzymatic aminohydroxylation of β-methylstyrene affords 1<I>R</I>,2<I>R</I> and 1<I>S</I>,2<I>R</I>-phenylpropanolamines in excellent chemical and optical purity by consuming dioxygen, ammonia and formate in aqueous buffer and ambient temperature.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c9gc03161h'/><BR/></P>