초록
<P><B>Abstract</B></P> <P>Nonsense mutation in the <I>bcsC</I> gene occurred in the ethanol-adapted strain of <I>Komagataeibacter oboediens</I> MSKU 3, E3 strain, resulting in the loss of the function to produce BNC. In this study, we tried to restore the BNC-producing ability of E3 strain by the following adaptive mutation through repetitive static culture, and obtained four BNC-producing revertant strains, of which the <I>bcsC</I> gene had InDel mutations near the frameshift mutation region in E3 strain, resulting in several amino acid alterations compared with the BcsC of MSKU 3. Each revertant produced BNCs with different productivity on the static culture. Interestingly, one of the revertants, R37-9, produced BNC with a finer structure and narrower range of fibrils width, compared to others. The genome of R37-9 strain revealed only one amino acid substitution in the <I>bcsC</I> gene. Thus, we concluded that N713D mutation occurred in the <I>bcsC</I> gene is responsible for the finer fibrils structure.</P> <P><B>Highlights</B></P> <P> <UL> <LI> BNC producibility was restored by adaptation through repetitive static cultures. </LI> <LI> Different mutations in the <I>bcsCI</I> gene resulted in different BNC fibrils properties. </LI> <LI> N713D mutation is responsible for a finer and narrower range of BNC fibrils width. </LI> </UL> </P>