BioChem - DOS

Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

Chemical engineering journal

Hwang, Ho Won; Yoon, Jihee; Min, Kyoungseon; Kim, Min-Sik; Kim, Seung-Jin; Cho, Dae Haeng; Susila, Hadiyati; Na, Jeong-Geol; Oh, Min-Kyu; Kim, Yong Hwan

<P><B>Abstract</B></P> <P>Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, <I>Acetobacterium woodii</I>, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H<SUB>2</SUB>. The conversion yield and specificity were close to 100% when the strain&rsquo;s energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered <I>Methylbacterium extorquens</I> AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Carbon monoxide (CO) can be utilized as a substrate for biopolymer production. </LI> <LI> Whole-cell biocatalyst converted CO to formate with 100% yield and specificity. </LI> <LI> The formate was used for biopolymer production by an engineered microorganism. </LI> <LI> The two-stage bioconversion of CO to biopolymer is a novel and promising strategy. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2020

Elsevier

1385-8947

389

pp.124394

10.1016/j.cej.2020.124394

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98961604

Carbon monoxide; Formate; Poly-3-hydroxybutyrate; Two-stage bioconversion; Biopolymer