BioChem - DOS

Membrane-integrated fermentation system for improving the optical purity of D-lactic acid produced during continuous fermentation

Bioscience, biotechnology, and biochemistry

SAWAI, Hideki; NA, Kyungsu; SASAKI, Nanami; MIMITSUKA, Takashi; MINEGISHI, Shin-ichi; HENMI, Masahiro; YAMADA, Katsushige; SHIMIZU, Sakayu; YONEHARA, Tetsu

<P>This report describes the production of highly optically pure <SMALL>D</SMALL>-lactic acid by the continuous fermentation of <I>Sporolactobacillus laevolacticus</I> and <I>S. inulinus</I>, using a membrane-integrated fermentation (MFR) system. The optical purity of <SMALL>D</SMALL>-lactic acid produced by the continuous fermentation system was greater than that produced by batch fermentation; the maximum value for the optical purity of <SMALL>D</SMALL>-lactic acid reached 99.8% enantiomeric excess by continuous fermentation when <I>S. leavolacticus</I> was used. The volumetric productivity of the optically pure <SMALL>D</SMALL>-lactic acid was about 12 g/L/h, this being approximately 11-fold higher than that obtained by batch fermentation. An enzymatic analysis indicated that both <I>S. laevolacticus</I> and <I>S. inulinus</I> could convert <SMALL>L</SMALL>-lactic acid to <SMALL>D</SMALL>-lactic acid by isomerization after the late-log phase. These results provide evidence for an effective bio-process to produce <SMALL>D</SMALL>-lactic acid of greater optical purity than has conventionally been achieved to date.</P>

2011

Japan Society for Bioscience, Biotechnology, and Agrochemistry

0916-8451

1347-6947

75

12

pp.2326-2332

10.1271/bbb.110486

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58207640

continuous fermentation; D-lactic acid; optical purity; poly(lactic acid); isomerization