초록
<P><B>Abstract</B></P> <P>Two-stage anaerobic digestion of food waste was performed using four different inoculum pre-treatment methods to enrich hydrogen (H<SUB>2</SUB>) producing bacteria from sludge. The pretreatments used in this study included heat shock, alkaline treatment, aeration, and a novel pretreatment using waste frying oil (WFO). Alkaline pretreatment and aeration did not completely inhibit methanogens in the first stage while no methane (CH<SUB>4</SUB>) was detected in the reactors cultivated either with heat shock or WFO-pretreated inocula. The highest H<SUB>2</SUB> and CH<SUB>4</SUB> yields (76.1 and 598.2 mL/gVS, respectively) were obtained using the inoculum pretreated with WFO. The highest total energy yield (21.96 kJ/gVS) and total organic carbon (TOC) removal efficiencies (95.77%) were obtained using inoculum pretreatment with WFO. The total energy yield trend obtained using the different pretreatments was as follows: WFO > alkaline > heat > aeration > control.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Two-stage anaerobic digestion using a novel inoculum pre-treatment method. </LI> <LI> The novel pre-treatment was compared with three common inoculum pre-treatment methods. </LI> <LI> Highest H<SUB>2</SUB> and CH<SUB>4</SUB> productions were obtained using the novel pre-treatment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>