초록
<P><B>Abstract</B></P> <P>Inhibition of sodium ion (Na<I> <SUP>+</SUP> </I>) on <I>Zymomonas mobilis</I> represents an important obstacle for efficient cellulosic ethanol production. This study screened and overexpressed the genes responsible for transporting metal ions in <I>Z. mobilis</I> for increasing its Na<I> <SUP>+</SUP> </I> tolerance. The <I>ZMO0119</I> gene encoding Na<SUP>+</SUP>/H<SUP>+</SUP> antiporter was identified to be highly effective for reducing intracellular Na<SUP>+</SUP> concentration of <I>Z. mobilis</I> by improving the Na<I> <SUP>+</SUP> </I> transport capacity. Overexpression of <I>ZMO0119</I> gene in <I>Z. mobilis</I> significantly accelerated the cell growth, glucose consumption, and cellulosic ethanol production from the dry acid pretreated and biodetoxified corn stover feedstock. This study provided an important gene responsible for increasing the cellulosic ethanol fermentability by <I>Z. mobilis</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>ZMO0119</I> gene was highly responsible for improving Na<SUP>+</SUP> transport capacity. </LI> <LI> A sodium ion tolerant <I>Z. mobilis</I> recombinant was successfully developed. </LI> <LI> Ethanol fermentation from the pretreated corn stover was obviously accelerated. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>