초록
<P><B>Abstract</B></P> <P>In this study, biobutanol production from glucose, starch and food waste by newly identified <I>Clostridium</I> sp. strain HN4 was comprehensively investigated, which is capable of secreting amylase indigenously for the following acetone-butanol-ethanol fermentation. With pH adjustment, strain HN4 could produce 5.23 g/L of butanol from 60 g/L of starch with secretion of 1.95 U/mL amylase through consolidated bioprocessing. Further supplementation of 3 g/L of CaCO<SUB>3</SUB> and 0.5% non-ionic surfactant of Tween 80 could stimulate both amylase activities and the final butanol titer, leading to 17.64 g/L of butanol with yield of 0.15 g/g. Fed batch fermentation integrated with <I>in situ</I> removal could further improve the butanol titer to 35.63 g/L with yield of , representing the highest butanol production and yield from food waste. These unique features of <I>Clostridium</I> sp. strain HN4 could open the door to the possibility of cost-effective biobutanol production from food waste on a large scale.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An amylatic and solventogenic <I>Clostridium</I> sp. strain HN4 was isolated and characterized. </LI> <LI> CaCO<SUB>3</SUB> and Tween could significantly improve amylase activity and butanol titer. </LI> <LI> 17.64 g/L of butanol was obtained from 120 g/L food wastes. </LI> <LI> <I>In situ</I> removal could improve the final butanol titer to 35.63 g/L. </LI> </UL> </P>