BioChem - DOS

Efficient Chemo-Enzymatic Epoxidation Using silcoat-Novozym435: Characterizing the Multiphase System

Chemical engineering & technology

Bhattacharya, S.; Drews, A.; Lyagin, E.; Kraume, M.; Ansorge‐ Schumacher, M. B.

<P><B>Abstract</B></P><P>Silicone composites of Novozym&reg;435 (<I>silcoat</I>&#8208;NZ435) demonstrate improved leaching and mechanical stability compared to native Novozym&reg;435 (NZ435). Here, the application scope of this novel enzyme preparation has been broadened significantly by the transfer from single&#8208;phase esterification to a complex chemo&#8208;enzymatic epoxidation reaction in a three&#8208;phase system. The reaction gave up to 80&thinsp;% yields with almost 100&thinsp;% selectivity in 50&thinsp;h when operated in the absence of mass transfer limitations. Characterization of the system revealed that octanoic acid inhibited the reaction at concentrations around 6&thinsp;M. The <I>silcoat</I>&#8208;NZ435 catalyst could be reused over five days with less than 50&thinsp;% loss in activity when 5&thinsp;M H<SUB>2</SUB>O<SUB>2</SUB> was present. H<SUB>2</SUB>O<SUB>2</SUB> was also identified as the component which deactivates the enzyme at high concentration (17.5&thinsp;M) demanding spatial separation of the <I>silcoat</I>&#8208;NZ435 for optimal operation.</P>

2012

WILEY&#x2010;VCH Verlag

0930-7516

1521-4125

35

8

pp.1448-1455

10.1002/ceat.201100640

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63757457

Chemo&#x2010; enzymatic expoxidation; Enzymatic synthesis; Epoxide; Multiphase systems;