초록
Biological production of hydrogen was evaluated by introducing microphyte Chlorella vulgaris strain into acid hydrolysate (sulfuric acid) of Valoniopsis pachynema in anaerobic photo fermentation under sulfur deprivated condition. In batch fermentation process, low concentration of acid hydrolysate showed encouraging biomass production. The maximum level of cultural growth and photobiological hydrogen production was obtained at 30% acid hydrolysate on 8th day along with sustained production of biohydrogen up to 6 days. The optimization of substrate concentration and other significant variables (Temperature, pH and CO<SUB>2</SUB> concentration) were examined by Response Surface Methodology. The results revealed that the production of photobiological hydrogen was higher than the batch fermentation with maximum photobiological hydrogen production of about 0.0039 g h<SUP>-1</SUP> L<SUP>-1</SUP>, which were achieved at optimum conditions of substrate concentration (3.25 g L<SUP>-1</SUP>); temperature (32 <SUP>o</SUP>C), pH (7.66) and carbon dioxide concentration (3.96%). The results suggest that, acid hydrolysate of V. pachynema acts as an effective, inexpensive resource for culturing and acquiring biohydrogen from C. vulgaris strain.