초록
<P><I>Zymomonas mobilis</I> is an ethanologenic bacterium and is known to be an example microorganism with energy-uncoupled growth. A genome-scale metabolic model could be applicable for understanding the characteristics of <I>Z. mobilis</I> with rapid catabolism and inefficient energy conversion. In this study, a charge balanced genome-scale metabolic model (iEM439) of <I>Z. mobilis</I> ATCC 10988 (ZM1) including 439 genes, 692 metabolic reactions and 658 metabolites was reconstructed based on genome annotation and previously published information. The model presents a much better prediction for biomass and ethanol concentrations in a batch culture by using dynamic flux balance analysis compared with the two previous genome-scale metabolic models. Furthermore, intracellular flux distribution obtained from the model was consistent with the fluxes for glucose fermentation determined by <SUP>13</SUP>C NMR. The model predicts that there is no difference in growth rates of <I>Z. mobilis</I> under aerobic and anaerobic conditions whereas ethanol production is decreased and production of other metabolites including acetate and acetoin is increased under aerobic conditions. Experimental data confirm the predicted differences between the aerobic and anaerobic growth of <I>Z. mobilis</I>. Finally, the model was used to study the energy-uncoupled growth of <I>Z. mobilis</I> and to predict its effect on flux distribution in the central metabolism. Flux distribution obtained from the model indicates that coupling growth and energy reduces ethanol secretion and changes the flux distribution to produce more biomass. This coupling is also associated with a significant increase in the proton uptake rate based on the prediction of the charge balanced model. Hence, resistance to intracellular pH reduction could be the main reason for uncoupled growth and <I>Z. mobilis</I> uses ATPase to pump out the proton. Experimental observations are in accordance with the predicted relationship between growth, ATP dissipation and proton exchange.</P><BR><BR><P>Graphic Abstract</P><P><I>Zymomonas mobilis</I> is an ethanologenic bacterium and is known to be an example microorganism with energy-uncoupled growth. The reconstructed metabolic model indicate that resistance to intracellular pH reduction could be the main reason for uncoupled growth.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5mb00588d'><BR></P>