초록
The filamentous fungus Paecilomyces lilacinus was grown on n-hexadecane in submerged (SmC) and solid-state (SSC) cultures. The maximum CO<SUB>2</SUB> production rate in SmC (V<SUB>max</SUB>=11.7mgCO<SUB>2</SUB>L<SUB>g</SUB><SUP>-1</SUP>day<SUP>-1</SUP>) was three times lower than in SSC (V<SUB>max</SUB>=40.4mgCO<SUB>2</SUB>L<SUB>g</SUB><SUP>-1</SUP>day<SUP>-1</SUP>). The P. lilacinus hydrophobin (PLHYD) yield from the SSC was 1.3mgPLHYDgprotein<SUP>-1</SUP>, but in SmC, this protein was not detected. The PLHYD showed a critical micelle concentration of 0.45mgmL<SUP>-1</SUP>. In addition, the PLHYD modified the hydrophobicity of Teflon from 130.1+/-2<SUP>o</SUP> to 47+/-2<SUP>o</SUP>, forming porous structures with some filaments <1μm and globular aggregates <0.25μm diameter. The interfacial studies of this PLHYD could be the basis for the use of the protein to modify surfaces and to stabilize compounds in emulsions.