Enzymatic network for production of ether amines from alcohols

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논문

Enzymatic network for production of ether amines from alcohols

학술지

Biotechnology and bioengineering

저자명

Palacio, Cyntia M.; Crismaru, Ciprian G.; Bartsch, Sebastian; Navickas, Vaidotas; Ditrich, Klaus; Breuer, Michael; Abu, Rohana; Woodley, John M.; Baldenius, Kai; Wu, Bian; Janssen, Dick B.

초록

ABSTRACTWe constructed an enzymatic network composed of three different enzymes for the synthesis of valuable ether amines. The enzymatic reactions are interconnected to catalyze the oxidation and subsequent transamination of the substrate and to provide cofactor recycling. This allows production of the desired ether amines from the corresponding ether alcohols with inorganic ammonium as the only additional substrate. To examine conversion, individual and overall reaction equilibria were established. Using these data, it was found that the experimentally observed conversions of up to 60% observed for reactions containing 10 mM alcohol and up to 280 mM ammonia corresponded well to predicted conversions. The results indicate that efficient amination can be driven by high concentrations of ammonia and may require improving enzyme robustness for scale‐up. Biotechnol. Bioeng. 2016;113: 1853–1861. © 2016 Wiley Periodicals, Inc.

발행연도

2016

ISSN

0006-3592

ISSN

1097-0290

권

113

호

페이지

pp.1853-1861

DOI