Immobilization of lipase in hierarchically ordered macroporous/mesoporous silica with improved catalytic performance

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바이오화학분류

- 바이오플라스틱
  1. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
  3. 연료
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 식품첨가제

논문

Immobilization of lipase in hierarchically ordered macroporous/mesoporous silica with improved catalytic performance

학술지

Journal of molecular catalysis. B, Enzymatic

저자명

Jiang, Y.; Zheng, P.; Zhou, L.; Kong, W.; Gao, J.; Wang, J.; Gu, J.; Zhang, X.; Wang, X.

초록

Hierarchically ordered macroporous/mesoporous silica (3DOM/m-S) material was prepared through the dual templating method with polystyrene (PS) colloidal crystals as the hard template and amphiphilic triblock copolymers (P123) as the soft template. The achieved 3DOM/m-S possesses ordered macropores of 400nm and mesopores of 5.1nm, which provides a promising platform for enzyme immobilization. Lipase B from Candida antarctica (CALB) was employed as a model enzyme to verify the possibility and advantages of enzyme immobilized on 3DOM/m-S. The immobilized lipase shows excellent stability towards heat even at 80<SUP>o</SUP>C and organic solvents for long-term incubation (288h). Also, the immobilized CALB could be used for esterification reactions between acids and alcohols with different chain lengths, and 90% of conversion rate could be reached. In examining the reusability in esterification of oleic acid and ethanol, the conversion rate can retain 75% after 10 reaction cycles, indicating a remarkable reusability of the immobilized lipase.

발행연도

2016

발행기관

Elsevier

ISSN

1381-1177

ISSN

1873-3158

권

130

페이지

pp.96-103