Biocascade synthesis of L-tyrosine derivatives by coupling a thermophilic tyrosine phenol-lyase and L-lactate oxidase

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논문

Biocascade synthesis of L-tyrosine derivatives by coupling a thermophilic tyrosine phenol-lyase and L-lactate oxidase

학술지

European journal of organic chemistry

저자명

Li, Guosi; Lian, Jiazhang; Xue, Hailong; Jiang, Yiqi; Ju, Shuyun; Wu, Mianbin; Lin, Jianping; Yang, Lirong

초록

A one‐pot biocascade of two enzymatic steps catalyzed by an l‐lactate oxidase and a tyrosine phenol‐lyase has been successfully developed in the present study. The reaction provides an efficient method for the synthesis of l‐tyrosine derivatives, which exhibits readily available starting materials and excellent yields. In the first step, an in situ generation of pyruvate from readily available bio‐based l‐lactate catalyzed by a highly active l‐lactate oxidase from Aerococcus viridans (AvLOX) was developed (using oxygen as oxidant and catalase as hydrogen peroxide removing reagent). Pyruvate thus produced underwent C–C coupling with phenol derivatives as acceptor substrate using specially designed thermophilic tyrosine phenol‐lyase mutants from Symbiobacterium toebii (TTPL). Overall, this cascade avoids the high cost and easy decomposition of pyruvate and offered an efficient and environmentally friendly procedure for l‐tyrosine derivatives synthesis.

발행연도

2020

ISSN

1434-193x

ISSN

1099-0690

권

2020

호

페이지

pp.1050-1054

DOI