BioChem - DOS

Assessing the impact of palmitic, myristic and lauric acids on hydrogen production from glucose fermentation by mixed anaerobic granular cultures

International journal of hydrogen energy

Saady, N.M.C.; Chaganti, S.R.; Lalman, J.A.; Veeravalli, S.S.; Shanmugam, S.R.; Heath, D.D.

The effects of lauric (LUA), myristic (MA), palmitic (PA), and a mixture of myristic:palmitic (MA:PA) acids on hydrogen (H<SUB>2</SUB>) production from glucose degradation using anaerobic mixed cultures were assessed at 37 <SUP>o</SUP>C with an initial pH set at 5.0 and 7.131 mM of each acid. The maximum H<SUB>2</SUB> yield (2.53 +/- 0.18 mol mol<SUP>-1</SUP> glucose) was observed in cultures treated with PA. A principal component analysis (PCA) of the by-products and the microbial population data sets detected similarities between the controls and PA treated cultures; however, differences were observed between the controls and PA treated cultures in comparison to the MA and LUA treated cultures. The flux balance analysis (FBA) showed that PA decreased the quantity of H<SUB>2</SUB> consumed via homoacetogenesis compared to the other LCFAs. The control culture was dominated by Thermoanaerovibrio acidaminovorans (60%), Geobacillus sp. and Eubacterium sp. (28%), while Clostridium sp. was less than 1%. Treatment with PA, MA, MA:PA, or LUA increased the H<SUB>2</SUB> producers (Clostridium sp. and Bacillus sp.) population by approximately 48, 67, 86, and 86%, respectively.

2012

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

37

24

pp.18761-18772

10.1016/j.ijhydene.2012.09.116

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART64954897

Long chain fatty acids; Flux balance analysis; Principal component analysis; Mixed anaerobic cultures; Hydrogen; Microbial diversity