초록
<P>This study investigated the effects of initial pHs and temperatures to the hydrogen production ration and cell growth of<I>Biohydrogenbacterium</I>R3<I>sp.nov</I>.. The initial pHs were set at 4.0, 4.5, 5.0, 5.5, 6.0, 6.5 and 6.7, respectively and the temperatures were increased from 25 °C to 45 °C in regular intervals of 5 °C at 10 g/L of the initial glucose concentration. The results indicated that pH value had a noticeable effect on the cell growth and hydrogen production. The dry cell weight and hydrogen production yield got the maximum of 0.6308 g/L and 34.2 mmol/L, respectively when the initial pH was 5.5. The final pH in the culture were always kept at 3.0~4.0. Temperature is also known to affect the maximum cell growth and specific hydrogen production ration (SHPR). And they got the maximum of 0.6682 g/L and 1.0145mol H2/mol glucose, respectively when the temperature was 30 °C. It is obvious that hydrogen production and biomass will be inhibited gradually with increasing this pH and temperature or decreasing it.</P>