Sequential acid and enzymatic saccharification of steam exploded cotton stalk and subsequent ethanol production using Scheffersomyces stipitis NCIM 3498
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Sequential acid and enzymatic saccharification of steam exploded cotton stalk and subsequent ethanol production using Scheffersomyces stipitis NCIM 3498
<P><B>Abstract</B></P> <P>The present study was mainly focused on effective utilization of holocellulose content of cotton stalk for bioethanol production. Steam exploded cotton stalk (180 °C for 5 min; severity factor- 3.05) was subjected to sequential acid and enzymatic saccharification. Acid saccharification (1.5% H<SUB>2</SUB>SO<SUB>4</SUB> at 110 °C for 60 min) of steam exploded cotton stalk released maximum of 37.24 ± 1.20 g/L reducing sugars with 69.16 ± 1.18% holocellulose hydrolysis. Subsequent enzymatic saccharification of steam exploded acid treated residue yielded maximum 8.50 ± 0.57 g/L sugar concentration with 84.20 ± 0.34% combined saccharification efficiency using 20 FPU/g cellulase loading (Accellerase- 1500) after 72 h incubation period. Finally, batch fermentation of mixed (acid-enzymatic) hydrolysate containing 45.74 ± 1.68 g/L sugars with <I>Scheffersomyces stipitis</I> NCIM 3498 produced 19.08 ± 0.56 g/L ethanol with 0.47 g/g ethanol yield after 48 h time period.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Steam exploded CS can be a potential substrate for ethanol production. </LI> <LI> Sequential acid and enzymatic saccharification showed promising sugar yield. </LI> <LI> Mixed hydrolysate with <I>S. stipitis</I> strain produced 0.47 g/g ethanol yield. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>