BioChem - DOS

Using tobacco waste extract in pre-culture medium to improve xylose utilization for L-lactic acid production from cellulosic waste by Rhizopus oryzae

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zheng, Y.; Wang, Y.; Zhang, J.; Pan, J.

The aim of this work was to study the high-titer l-lactic acid production from cellulosic waste using Rhizopus oryzae. The tobacco waste water-extract (TWE) added with 5g/L glucose and 0.1g/L vitamin C was optimized as pre-culture medium for R. oryzae. Results found that compared to traditional pre-culture medium, it improved xylose consumption rate up to 2.12-fold and enhanced l-lactic acid yield up to 1.73-fold. The highest l-lactic acid concentration achieved was 173.5g/L, corresponding to volumetric productivity of 1.45g/Lh and yield of 0.860g/g total reducing sugar in fed-batch fermentation. This process achieves efficient production of polymer-grade l-lactic acid from cellulosic feedstocks, lowers the cost of fungal cell pre-culture and provides a novel way for re-utilization of tobacco waste.

2016

Elsevier Applied Science

0960-8524

218

pp.344-350

10.1016/j.biortech.2016.06.071

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART76147088

Tobacco waste; l-Lactic acid; Rhizopus oryzae; Xylose; Pre-culture