초록
A two-stage modelling and optimization of biohydrogen production is reported. A mixture design was used to determine the optimum proportions of bean husk (BH), corn stalk (CS), and organic fraction of solid municipal waste (OFSMW). The optimum operational setpoints for substrate concentration, pH, temperature and hydraulic retention time (HRT) were further investigated using the box-behnken design. The quadratic polynomial model from the mixture design had a coefficient of determination (R<SUP>2</SUP>) of 0.9427 and the optimized mixtures were in the ratio of OFSMW:BH:CS = 30:0:0 and OFSMW:BH:CS = 15:15:0 with yields of 56.47 ml H<SUB>2</SUB>/g TVS and 41.16 ml H<SUB>2</SUB>/g TVS respectively. Optimization on physico-chemical process parameters on the improved substrate gave the setpoints of 40.45 g/l, 7.9, 30.29 <SUP>o</SUP>C, 86.28 h for substrate concentration, pH, temperature and HRT respectively having a predicted H<SUB>2</SUB> yield of 57.73 ml H<SUB>2</SUB>/g TVS. Model validation gave 58.62 ml H<SUB>2</SUB>/g TVS, thus an improvement of 3.8% on the optimized mixture. Biohydrogen production can be significantly enhanced by a suitable mixture of agro-municipal waste and operational optimal setpoints.