Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
기타
바이오정밀화학
용매
화학제품
연료
기타
화장품용 기능성소재
계면활성제⁄증점제
기타
의료용 화학소재
식품첨가제
논문
Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase
<P><B>Highlights</B></P><P>► <I>Thermoanaerobacter brockii</I> β-glucosidase (CglT) enhanced cellulolytic activity of cellulosomes. ► Cellulosome–CglT system was very efficient in rice straw hydrolysis. ► Required cellulosome–CglT loading was much lower than <I>Trichoderma reesei</I> cellulase system. ► Ammonia treated rice straw hydrolysate was fermented to ethanol without inhibition.</P> <P><B>Abstract</B></P><P><I>Clostridium thermocellum</I> is known to produce the cellulosomes with efficient plant cell wall degradation ability. To bring out the maximum cellulolytic ability of the cellulosomes, it is necessary to eliminate the end product inhibition by cellobiose. Combinations of β-glucosidases from thermophilic anaerobic bacteria and <I>Aspergillus</I> <I>niger</I> and <I>C.</I> <I>thermocellum</I> S14 cellulosomes were evaluated for optimization of cellulose degradation. β-Glucosidase (CglT) from <I>Thermoanaerobacter</I> <I>brockii</I>, in combination with cellulosomes, exhibited remarkable saccharification ability for microcrystalline cellulose. When rice straw, soaked in 28% aqueous ammonia for 7days at 60°C, was hydrolyzed by an enzyme loading combination of 2mg cellulosome and 10 units CglT per g glucan, 91% of glucan was hydrolyzed to glucose, indicating roughly1/10 the enzyme load of a <I>Trichoderma</I> <I>reesei</I> cellulase (Celluclast 1.5L) and Novozyme-188 combination is enough for the combination of <I>C.</I> <I>thermocellum</I> S14 cellulosomes and CglT to achieve the same level of saccharification of rice straw.</P>