초록
<P>Organic solvent-tolerant lipases have shown new potential application in biotechnological fields. A novel strain which produced an organic solvent-tolerant lipase was isolated from soils in different areas of China. Based on the morphological, physiological and biochemical features and 16S rDNA sequence of the strain, it was identified as<I>Pseudomonas aeruginosa</I>CS-2. The crude lipase had an improved stability in the presence of acetonitrile and it exhibited stability in presence of benzene, chloroform,<I>n</I>-Hexane, petroleum ether and isooctane. The optimized composition of culture medium was as follows: olive oil 1%, peptone 4%, K2HPO40.5%, MgSO4·7H2O 0.1%, gum arabic 0.04%. The optimum condition for lipase production was on the following: temperature 37°C, initial pH 7.5, rotary shaking speed 200 rev/min, culture time 48 h.</P>