Boosting D-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

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- 바이오플라스틱
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- 바이오정밀화학
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논문

Boosting D-lactate production in engineered cyanobacteria using sterilized anaerobic digestion effluents

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Hollinshead, W.D.; Varman, A.M.; You, L.; Hembree, Z.; Tang, Y.J.

초록

Anaerobic digestion (AD) is an environmentally friendly approach to waste treatment, which can generate N and P-rich effluents that can be used as nutrient sources for microalgal cultivations. Modifications of AD processes to inhibit methanogenesis leads to the accumulation of acetic acid, a carbon source that can promote microalgal biosynthesis. This study tested different AD effluents from municipal wastes on their effect on d-lactate production by an engineered Synechocystis sp. PCC 6803 (carrying a novel lactate dehydrogenase). The results indicate that: (1) AD effluents can be supplemented into the modified BG-11 culture medium (up to 1:4 volume ratio) to reduce N and P cost; (2) acetate-rich AD effluents enhance d-lactate synthesis by ~40% (1.2g/L of d-lactate in 20days); and (3) neutral or acidic medium had a deleterious effect on lactate secretion and biomass growth by the engineered strain. This study demonstrates the advantages and guidelines in employing wastewater for photomixotrophic biosynthesis using engineered microalgae.

발행연도

2014

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

169

페이지

pp.462-467

DOI

10.1016/j.biortech.2014.07.003